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KMID : 0613820060160020328
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Journal of Life Science
2006 Volume.16 No. 2 p.328 ~ p.332
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Inhibitory Effect of Thujae orientalis Semen Extract on Pancreatic Lipase Activity
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Kim Min-Soo
Kim Bo-Yeon
Park Chan-Sun
Ahn Soon-Cheol
Oh Won-Keun
Ahn Jong-Seog
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Abstract
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The possible presence of inhibitors of pancreatic lipase (tricaylglycerol acylhydrolase EC 3.1.1.3) was screened from Korean traditional edible or medicinal herbs. Among tested herbs, Arecae pericarpium, Mucunae Caulis, Rhus javanica, Thujae orientalis were shown to have strong inhibitory effect against pancreatic lipase. Thujae orientalis was finally selected as a candidate for pancreatic lipase inhibitor. The extract of Thujae orientalis was showed selective inhibition on porcine pancreatic lipase activity. Active inhibitors, TF-1, TF-2, TF-3, were purified from an extract of Thujae orientalis, using chloroform extraction, followed by successive chromatography in silica gel and LH-20 and high performance liquid chromatography (HPLC). The IC50 values of TF-1, TF-2, TF-3 and orlistat were 44.7, 98.7, 46.1 and 27.6 ㎍/㎖, respectively. And also the TF-2 and orlistat were shown to be inhibitory effect on the differentiation of preadipocyte NIH-3T3 L1 cells at a concentration of 10 ㎍/㎖.
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KEYWORD
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Pancreatic lipase inhibitor, Thujae orientalis
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